目的 探索马兰草活性部位的量效关系及谱效关系。方法 设置不同剂量组,观测抗炎活性之间的差异性,同时,测定不同剂量组的高效液相色谱指纹图谱,利用统计学方法,计算其图谱和药效之间的关系。结果 不同剂量马兰草乙醇提取物与抗炎活性之间呈正相关性,相关系数为0.951 1;HPLC指纹图谱显示马兰草乙醇提取物有18个共有峰,指认了其中4个,各共有峰与抗炎活性之间的综合关联度在0.560 8~0.959 9之间,关联度大小顺序为:10>14>5>8>15> 9>2>7>12>1 >17 >3 >18>16> 6>13>4>11。结论 所建立的马兰草 HPLC 特征图谱与药效作用的关系,可为建立指纹图谱与药效关联的中药质量控制模式提供理论依据和数据支持。
Abstract
ObjectiveTo explore the dose-effect and fingerprint-effect relationships of the active site of Kalimeris indica. Methods The HPLC fingerprints of the active site at different doses were determined. Mouse auricle swelling model was used to observe the anti-inflammatory action of Kalimeris indica extract and its dose-response relationship. Grey correlation method was used to assess the relationship between the fingerprint peaks and effects. Results The was a positive correlation between the dose and anti-inflammatory activity of the extract (r=0. 9511). The extract had 18 common peaks, among which four were identified, and the comprehensive correlation between the common peaks and anti-inflammatory activity was from 0.560 8 to 0.959 9, with ranking of 10>14>5>8>15>9>2>7>12>1>17>3>18>16>6>13>4>11. Conclusion The spectrum-activity relationship of Kalimeris indica established in this study could provide theoretical basis and data support for the quality control pattern of traditional Chinese medicines.
关键词
马兰草 /
抗炎活性 /
高效液相色谱法 /
指纹图谱法 /
谱效关系 /
原儿茶酸 /
丁香酸
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Key words
Kalimeris indica /
anti-inflammatory activity /
HPLC /
Fingerprint /
Spectrum-activity relationship
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中图分类号：
R284.2
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参考文献
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JIANG R B. Chemical component and fingerprint research for Kalimeris indica.Guizhou Normal University (贵州师范大学),2012.
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脚注
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基金
基金项目:贵州省中药现代化科技产业研究开发专项资助项目(黔科合中药字5020);贵阳市科学技术计划资助项目(筑科合农合同字第1-中-22号);贵州省科技创新人才团队建设资助项目(黔科合人才团队4008); 贵州省教育厅特色重点实验室资助项目(黔科合KY005号)
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